Reproduction Abstracts

Little is known about the involvement of miRNAs in luteal development. Cattle provide a convenient model to study ovarian physiology in monovular species. Our previous microarray studies in bovine showed significant upregulation of miR-96 and miR-132 in luteal relative to follicular tissues. In the present study we used an in vitro model of forskolin-induced follicular granulosa cell luteinisation, transfection with specific locked nucleic acid inhibitors or mimics of...

Little is known about the involvement of miRNAs during terminal follicle differentiation in the monovular ovary. This study aimed to characterise miRNAs involved in the follicle-luteal transition in bovine. Microarray analyses were performed on RNA from ovulatory-size follicles (n=6) and early corpora lutea (n=6) obtained at an abattoir. Exiqon’s miRCURY LNA microRNA Array, sixth generation was used and results were validated by qPCR. A total of ten and ...